BLASTN 2.11.0+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: Diaci_v3.0.ref.unplaced.fa 23,672 sequences; 201,546,927 total letters Query= 1 Length=2133 Score E Sequences producing significant alignments: (Bits) Value Scaffold_21533_22396 584 4e-165 Scaffold_21532_22395 551 4e-155 Scaffold_10660_11155 337 1e-90 Scaffold_24498_25463 183 2e-44 Scaffold_7203_7514 117 2e-24 Scaffold_2930_3051 84.2 2e-14 >Scaffold_21533_22396 Length=13900 Score = 584 bits (316), Expect = 4e-165 Identities = 318/319 (99%), Gaps = 0/319 (0%) Strand=Plus/Plus Query 1815 AGACTACCTCTATGTTTCAGGCGCCCCGGAGCTGCTTCAGATCGTCGAAACCTCCCGCTC 1874 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 12107 AGACTACCTCTATGTTTCAGGCGCCCCGGAGCTGCTTCAGATCGTCGAAACCTCCCGCTC 12166 Query 1875 CTCCCCCCGCAGCGGGGGCGGCACGGGGCCCTCCATTCTCAAAAAGCCGCCCCTCGAAAA 1934 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 12167 CTCCCCCCGCAGCGGGGGCGGCACGGGGCCCTCCATTCTCAAAAAGCCGCCCCTCGAAAA 12226 Query 1935 CAGTTCCATCCTGGTCCTTCAACCGCCGCACCGGTATCCTCCCATCGAGTTCCAGGACGT 1994 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 12227 CAGTTCCATCCTGGTCCTTCAACCGCCGCACCGGTATCCTCCCATCGAGTTCCAGGACGT 12286 Query 1995 CAAGATATCCTGCAGTGCGACTTTGCCGCGCGCGCCGCCCCCTCCCCGTTTGCCCGAGTC 2054 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 12287 GAAGATATCCTGCAGTGCGACTTTGCCGCGCGCGCCGCCCCCTCCCCGTTTGCCCGAGTC 12346 Query 2055 ACAACCGCTCCTGTCGGGGGGCGGCACACTGGGCCGGTACAAACCGCAGCACAAGACCAT 2114 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 12347 ACAACCGCTCCTGTCGGGGGGCGGCACACTGGGCCGGTACAAACCGCAGCACAAGACCAT 12406 Query 2115 TGACGAGCTGAAAGTGTGA 2133 ||||||||||||||||||| Sbjct 12407 TGACGAGCTGAAAGTGTGA 12425 Score = 374 bits (202), Expect = 1e-101 Identities = 204/205 (99%), Gaps = 0/205 (0%) Strand=Plus/Plus Query 1612 GGTTGTTCCCTGTTGATTCTGAACGTCCTGATATTCGCCGGCGTGTATTATCAGCGGGAT 1671 |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 11342 GGTTGTTCCCTGCTGATTCTGAACGTCCTGATATTCGCCGGCGTGTATTATCAGCGGGAT 11401 Query 1672 AAATCCCGTCAGGGCGGCTCAGGTCACCAGGACTCGGCGGGCAGTATACATAGCAACACG 1731 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 11402 AAATCCCGTCAGGGCGGCTCAGGTCACCAGGACTCGGCGGGCAGTATACATAGCAACACG 11461 Query 1732 AGCTCTAATGGGAGTCAGAACGGGGCGGGTATGATCAAGCAGCGCCGCCAGGAGAACGGT 1791 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 11462 AGCTCTAATGGGAGTCAGAACGGGGCGGGTATGATCAAGCAGCGCCGCCAGGAGAACGGT 11521 Query 1792 GGACCTATGTCTAATATTAGTGCAG 1816 ||||||||||||||||||||||||| Sbjct 11522 GGACCTATGTCTAATATTAGTGCAG 11546 Score = 267 bits (144), Expect = 2e-69 Identities = 144/144 (100%), Gaps = 0/144 (0%) Strand=Plus/Plus Query 1204 GATATGAAGCCCAAGCTGAAGAATCATTATCGGGCTCACAGACTCTCGTTCTGGCTGAAC 1263 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6765 GATATGAAGCCCAAGCTGAAGAATCATTATCGGGCTCACAGACTCTCGTTCTGGCTGAAC 6824 Query 1264 CTTGTTCCCGATCTACATAAACCTGGCTCAGATGATGTACCCCGCTCACATCATATGCTG 1323 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 6825 CTTGTTCCCGATCTACATAAACCTGGCTCAGATGATGTACCCCGCTCACATCATATGCTG 6884 Query 1324 GGAGATGATGATGAGATCAATGAA 1347 |||||||||||||||||||||||| Sbjct 6885 GGAGATGATGATGAGATCAATGAA 6908 Score = 267 bits (144), Expect = 2e-69 Identities = 148/150 (99%), Gaps = 0/150 (0%) Strand=Plus/Plus Query 1465 CAGCAGGCTTCAGCTGCCGTGGTGAAGGTGGCTCCGTCTAGCGGTAGAGATCTGCAGGAA 1524 ||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||| Sbjct 10753 CAGCAGGCTTCAGCTTCCGTGGTGAAGGTGGCTCCGTCTAGCGGTAGAGATCTGCAGGAA 10812 Query 1525 GATTTACCGGACGGATCTGCCGCAGCTTCAGGACAGGAGGATGGGTTTGCCGCCTATTCT 1584 |||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||| Sbjct 10813 GATTTACCGGACGGATCTGCCGCAGCTTCAGGACAGGAGGATGGATTTGCCGCCTATTCT 10872 Query 1585 ACCGCACTCAGCGTCACCGTGGCCATTGGT 1614 |||||||||||||||||||||||||||||| Sbjct 10873 ACCGCACTCAGCGTCACCGTGGCCATTGGT 10902 Score = 222 bits (120), Expect = 4e-56 Identities = 124/126 (98%), Gaps = 0/126 (0%) Strand=Plus/Plus Query 1079 GGAACCCTAACGATGCTCAAGATGGTGGAGATATGGTACACAACACAGTGAGAGCAGAGA 1138 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4727 GGAACCCTAACGATGCTCAAGATGGTGGAGATATGGTACACAACACAGTGAGAGCAGAGA 4786 Query 1139 GGACCAAATTCAAGAACATCGAGTGGACAGCTTATGAGAGTGTACATAAGAAATATCTAA 1198 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | Sbjct 4787 GGACCAAATTCAAGAACATCGAGTGGACAGCTTATGAGAGTGTACATAAGAAATATCTCA 4846 Query 1199 GCCTGG 1204 | |||| Sbjct 4847 GTCTGG 4852 Score = 222 bits (120), Expect = 4e-56 Identities = 120/120 (100%), Gaps = 0/120 (0%) Strand=Plus/Plus Query 1348 ATTCCTCACTCCACTTCTTCTCCGGGTAAGCATCAAGCCCCACCCTCCACATATCAGAAC 1407 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7406 ATTCCTCACTCCACTTCTTCTCCGGGTAAGCATCAAGCCCCACCCTCCACATATCAGAAC 7465 Query 1408 TACGGACCCTCTGCCTCACATAACCTAGTGAATTTAACAAGCAATGATATTATACATCAG 1467 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7466 TACGGACCCTCTGCCTCACATAACCTAGTGAATTTAACAAGCAATGATATTATACATCAG 7525 >Scaffold_21532_22395 Length=19303 Score = 551 bits (298), Expect = 4e-155 Identities = 313/319 (98%), Gaps = 5/319 (2%) Strand=Plus/Plus Query 1815 AGACTACCTCTATGTTTCAGGCGCCCCGGAGCTGCTTCAGATCGTCGAAACCTCCCGCTC 1874 ||||||||||||||||||||||| ||||||||||||||||||||||||||||| |||||| Sbjct 12365 AGACTACCTCTATGTTTCAGGCG-CCCGGAGCTGCTTCAGATCGTCGAAACCT-CCGCTC 12422 Query 1875 CTCCCCCCGCAGCGGGGGCGGCACGGGGCCCTCCATTCTCAAAAAGCCGCCCCTCGAAAA 1934 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 12423 CTCCCCCCGCAGCGGGGGCGGCACGGGGCCCTCCATTCTCAAAAAGCCGCCCCTCGAAAA 12482 Query 1935 CAGTTCCATCCTGGTCCTTCAACCGCCGCACCGGTATCCTCCCATCGAGTTCCAGGACGT 1994 |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||| Sbjct 12483 CAGTTCCATCCTGGTCCTTCAACCGCCGCACCGGTATCCT--CATCGAGTTCCAGGACGT 12540 Query 1995 CAAGATATCCTGCAGTGCGACTTTGCCGCGCGCGCCGCCCCCTCCCCGTTTGCCCGAGTC 2054 |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||| Sbjct 12541 GAAGATATCCTGCAGTGCGACTTTGCCGCGCGCGCCGCCCCCT-CCCGTTTGCCCGAGTC 12599 Query 2055 ACAACCGCTCCTGTCGGGGGGCGGCACACTGGGCCGGTACAAACCGCAGCACAAGACCAT 2114 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 12600 ACAACCGCTCCTGTCGGGGGGCGGCACACTGGGCCGGTACAAACCGCAGCACAAGACCAT 12659 Query 2115 TGACGAGCTGAAAGTGTGA 2133 ||||||||||||||||||| Sbjct 12660 TGACGAGCTGAAAGTGTGA 12678 Score = 374 bits (202), Expect = 1e-101 Identities = 204/205 (99%), Gaps = 0/205 (0%) Strand=Plus/Plus Query 1612 GGTTGTTCCCTGTTGATTCTGAACGTCCTGATATTCGCCGGCGTGTATTATCAGCGGGAT 1671 |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 11602 GGTTGTTCCCTGCTGATTCTGAACGTCCTGATATTCGCCGGCGTGTATTATCAGCGGGAT 11661 Query 1672 AAATCCCGTCAGGGCGGCTCAGGTCACCAGGACTCGGCGGGCAGTATACATAGCAACACG 1731 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 11662 AAATCCCGTCAGGGCGGCTCAGGTCACCAGGACTCGGCGGGCAGTATACATAGCAACACG 11721 Query 1732 AGCTCTAATGGGAGTCAGAACGGGGCGGGTATGATCAAGCAGCGCCGCCAGGAGAACGGT 1791 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 11722 AGCTCTAATGGGAGTCAGAACGGGGCGGGTATGATCAAGCAGCGCCGCCAGGAGAACGGT 11781 Query 1792 GGACCTATGTCTAATATTAGTGCAG 1816 ||||||||||||||||||||||||| Sbjct 11782 GGACCTATGTCTAATATTAGTGCAG 11806 Score = 267 bits (144), Expect = 2e-69 Identities = 144/144 (100%), Gaps = 0/144 (0%) Strand=Plus/Plus Query 1204 GATATGAAGCCCAAGCTGAAGAATCATTATCGGGCTCACAGACTCTCGTTCTGGCTGAAC 1263 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7031 GATATGAAGCCCAAGCTGAAGAATCATTATCGGGCTCACAGACTCTCGTTCTGGCTGAAC 7090 Query 1264 CTTGTTCCCGATCTACATAAACCTGGCTCAGATGATGTACCCCGCTCACATCATATGCTG 1323 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7091 CTTGTTCCCGATCTACATAAACCTGGCTCAGATGATGTACCCCGCTCACATCATATGCTG 7150 Query 1324 GGAGATGATGATGAGATCAATGAA 1347 |||||||||||||||||||||||| Sbjct 7151 GGAGATGATGATGAGATCAATGAA 7174 Score = 259 bits (140), Expect = 3e-67 Identities = 147/150 (98%), Gaps = 1/150 (1%) Strand=Plus/Plus Query 1465 CAGCAGGCTTCAGCTGCCGTGGTGAAGGTGGCTCCGTCTAGCGGTAGAGATCTGCAGGAA 1524 ||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||| Sbjct 11012 CAGCAGGCTTCAGCTTCCGTGGTGAAGGTGGCTCCGTCTAGCGGTAGAGATCTGCAGGAA 11071 Query 1525 GATTTACCGGACGGATCTGCCGCAGCTTCAGGACAGGAGGATGGGTTTGCCGCCTATTCT 1584 |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||| Sbjct 11072 GATTTACCGGACGGATCTGCCGCAGCTTCAGGACAGGAGGATGGA-TTGCCGCCTATTCT 11130 Query 1585 ACCGCACTCAGCGTCACCGTGGCCATTGGT 1614 |||||||||||||||||||||||||||||| Sbjct 11131 ACCGCACTCAGCGTCACCGTGGCCATTGGT 11160 Score = 222 bits (120), Expect = 4e-56 Identities = 124/126 (98%), Gaps = 0/126 (0%) Strand=Plus/Plus Query 1079 GGAACCCTAACGATGCTCAAGATGGTGGAGATATGGTACACAACACAGTGAGAGCAGAGA 1138 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4996 GGAACCCTAACGATGCTCAAGATGGTGGAGATATGGTACACAACACAGTGAGAGCAGAGA 5055 Query 1139 GGACCAAATTCAAGAACATCGAGTGGACAGCTTATGAGAGTGTACATAAGAAATATCTAA 1198 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | Sbjct 5056 GGACCAAATTCAAGAACATCGAGTGGACAGCTTATGAGAGTGTACATAAGAAATATCTCA 5115 Query 1199 GCCTGG 1204 | |||| Sbjct 5116 GTCTGG 5121 Score = 222 bits (120), Expect = 4e-56 Identities = 120/120 (100%), Gaps = 0/120 (0%) Strand=Plus/Plus Query 1348 ATTCCTCACTCCACTTCTTCTCCGGGTAAGCATCAAGCCCCACCCTCCACATATCAGAAC 1407 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7667 ATTCCTCACTCCACTTCTTCTCCGGGTAAGCATCAAGCCCCACCCTCCACATATCAGAAC 7726 Query 1408 TACGGACCCTCTGCCTCACATAACCTAGTGAATTTAACAAGCAATGATATTATACATCAG 1467 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 7727 TACGGACCCTCTGCCTCACATAACCTAGTGAATTTAACAAGCAATGATATTATACATCAG 7786 >Scaffold_10660_11155 Length=26552 Score = 337 bits (182), Expect = 1e-90 Identities = 186/188 (99%), Gaps = 0/188 (0%) Strand=Plus/Plus Query 740 ACAGGTATCACCTGTCGGAGATTCTGGCCACGATCATCAACGAGTACACGGACTGGGAGC 799 |||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||| Sbjct 2961 ACAGGTATCACCTGTCCGAGATTCTGGCCACGATCATCAACGAGTACACGGACTGGGAGC 3020 Query 800 GTCCGGTGCAACATCCGGTCAACATTCGCGATGAAACACTAGAAGCGTTATCGGATGCGA 859 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3021 GTCCGGTGCAACATCCGGTCAACATTCGCGATGAAACACTAGAAGCGTTATCGGATGCGA 3080 Query 860 TGGTGGTGGCACCTAGCGTCAACACGGCGGACTTGCATTCGTCCGCGAGACGAAACTCGT 919 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | Sbjct 3081 TGGTGGTGGCACCTAGCGTCAACACGGCGGACTTGCATTCGTCCGCGAGACGAAACTCCT 3140 Query 920 ACCTGTAC 927 |||||||| Sbjct 3141 ACCTGTAC 3148 Score = 267 bits (144), Expect = 2e-69 Identities = 150/153 (98%), Gaps = 0/153 (0%) Strand=Plus/Plus Query 928 AGACAGGGCTGTATCCATGGAGAGGAACTGCCCTATCTGTTCGGGGCACCCTTGGTGGGA 987 |||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||| Sbjct 17937 AGACAGGGCTGTATCCATGGGGAGGAACTGCCCTATCTGTTCGGGGCACCCTTGGTGGGA 17996 Query 988 GGGGTCAACCATTTCCCCCGCAACTTCACCAAGTCTGAAATCCTCCTGAGTGAAGCCACC 1047 || |||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 17997 GGATTCAACCATTTCCCCCGCAACTTCACCAAGTCTGAAATCCTCCTGAGTGAAGCCACC 18056 Query 1048 ATGATCTATTTCAGCAACTTCGCCAGAACTGGG 1080 ||||||||||||||||||||||||||||||||| Sbjct 18057 ATGATCTATTTCAGCAACTTCGCCAGAACTGGG 18089 Score = 233 bits (126), Expect = 2e-59 Identities = 126/126 (100%), Gaps = 0/126 (0%) Strand=Plus/Plus Query 1079 GGAACCCTAACGATGCTCAAGATGGTGGAGATATGGTACACAACACAGTGAGAGCAGAGA 1138 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 25364 GGAACCCTAACGATGCTCAAGATGGTGGAGATATGGTACACAACACAGTGAGAGCAGAGA 25423 Query 1139 GGACCAAATTCAAGAACATCGAGTGGACAGCTTATGAGAGTGTACATAAGAAATATCTAA 1198 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 25424 GGACCAAATTCAAGAACATCGAGTGGACAGCTTATGAGAGTGTACATAAGAAATATCTAA 25483 Query 1199 GCCTGG 1204 |||||| Sbjct 25484 GCCTGG 25489 >Scaffold_24498_25463 Length=2209 Score = 183 bits (99), Expect = 2e-44 Identities = 99/99 (100%), Gaps = 0/99 (0%) Strand=Plus/Plus Query 1079 GGAACCCTAACGATGCTCAAGATGGTGGAGATATGGTACACAACACAGTGAGAGCAGAGA 1138 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 2111 GGAACCCTAACGATGCTCAAGATGGTGGAGATATGGTACACAACACAGTGAGAGCAGAGA 2170 Query 1139 GGACCAAATTCAAGAACATCGAGTGGACAGCTTATGAGA 1177 ||||||||||||||||||||||||||||||||||||||| Sbjct 2171 GGACCAAATTCAAGAACATCGAGTGGACAGCTTATGAGA 2209 >Scaffold_7203_7514 Length=21338 Score = 117 bits (63), Expect = 2e-24 Identities = 119/147 (81%), Gaps = 0/147 (0%) Strand=Plus/Minus Query 16 AACTACGGGCTGATGGACCAAATAGCGGCCCTCCATTGGATCCAGGAGAACATAGGGTAC 75 ||||||||||||||||||||||||||||||||||||||| | ||| |||| || | Sbjct 7533 AACTACGGGCTGATGGACCAAATAGCGGCCCTCCATTGGGTGCAGCAGAATATCGCCCTG 7474 Query 76 TTCAATGGGGACCCGTCCAATGTGACCCTGGTGGGGCATGGAACAGGCGCGGCCTGCGTC 135 || || |||||| ||||||||| || |||| || || || || ||||||||| | Sbjct 7473 TTTGGCGGCGACCCGGGAAATGTGACCATGATGGGACACGGTACGGGGGCGGCCTGCATT 7414 Query 136 AACTTCCTCATGATTTCGCCCGCCGTG 162 ||||||||||||||||||||| ||||| Sbjct 7413 AACTTCCTCATGATTTCGCCCACCGTG 7387 >Scaffold_2930_3051 Length=12532 Score = 84.2 bits (45), Expect = 2e-14 Identities = 114/147 (78%), Gaps = 5/147 (3%) Strand=Plus/Plus Query 16 AACTACGGGCTGATGGACCAAATAGCGGCCCTCCATTGGATCCAGGAGAACATAGGGTAC 75 |||| ||||||| ||||||||||||||||||||||||| || |||| || | Sbjct 1355 AACTTCGGGCTGCTGGACCAAATAGCGGCCCTCCATTG-----AGCAGAATATCGCCCTG 1409 Query 76 TTCAATGGGGACCCGTCCAATGTGACCCTGGTGGGGCATGGAACAGGCGCGGCCTGCGTC 135 || || |||||| ||||||||| || |||| || || || || ||||||||| | Sbjct 1410 TTTGGCGGCGACCCGGGAAATGTGACCATGATGGGACACGGTACGGGAGCGGCCTGCATT 1469 Query 136 AACTTCCTCATGATTTCGCCCGCCGTG 162 ||||||||||||||||||||| ||||| Sbjct 1470 AACTTCCTCATGATTTCGCCCACCGTG 1496 Score = 73.1 bits (39), Expect = 4e-11 Identities = 105/138 (76%), Gaps = 0/138 (0%) Strand=Plus/Plus Query 943 CATGGAGAGGAACTGCCCTATCTGTTCGGGGCACCCTTGGTGGGAGGGGTCAACCATTTC 1002 ||||| ||||| | ||||| | ||||| || ||| || | | ||| ||| ||||||| Sbjct 9165 CATGGTGAGGAGTTACCCTACATATTCGGTGCCCCCCTGATCGATGGGTTCAGCCATTTC 9224 Query 1003 CCCCGCAACTTCACCAAGTCTGAAATCCTCCTGAGTGAAGCCACCATGATCTATTTCAGC 1062 ||||| ||||||||||||||||| | ||||||||||||| ||| |||| | ||| Sbjct 9225 CCCCGGAACTTCACCAAGTCTGAGGTTGCCCTGAGTGAAGCCTTCATACTCTACCTGAGC 9284 Query 1063 AACTTCGCCAGAACTGGG 1080 ||||| || |||| ||| Sbjct 9285 AACTTTGCGCGAACAGGG 9302 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 422861053655 Database: Diaci_v3.0.ref.unplaced.fa Posted date: Jun 21, 2019 11:44 AM Number of letters in database: 201,546,927 Number of sequences in database: 23,672 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5