Imaging

Visualization of the psyllid, Diaphorina citri, which spreads the plant-infecting bacterium, in a 3D, rotational platform, that permits rapid dissections, analyses, and study of the internal and external anatomy of this psyllid has changed the way we look at insect structure and functions. Thus the high resolution scans produced combined with computer software can also produce new ways to link and associate information.


Psyllid Micro CT

The micro-CT videos shown below were published in Alba-Tercedor, J., Hunter, W. B., Cicero, J. M., Sainz-Barian, M. and Brown S. (2017). Use of micro-CT to elucidate details of the anatomy and feeding of the Asian Citrus Psyllid Diaphorina citri Kuwayama , 1908 (Insecta: Hemiptera , Lividae). In Bruker Micro-CT Users Meeting 2017 (pp. 270-285).

Micro CT study of the male adult of the Asian Citrus Psyllid (Diaphorina citri)




Micro-CT study of the Asian Citrus Psyllid (Diaphorina citri): feeding apparatus and stylets





Asian citrus psyllid stylet biogenesis

All Hemiptera shed their stylets with the exuviae during each molt to the next instar. New stylets are manufactured inside the head of the pharate instar to replace them, and, as the new instar lifts away from the exuviae, these are fitted into their functional positions so that feeding can be resumed.

The manufacture of new stylets (stylet biogenesis) is an extremely complex process that has been detailed in the potato psyllid. Transmission electron microscopy indicates that key features of this process are also present in the pharate adult Asian citrus psyllid. Therefore, this animation was crafted to simplify exposition of the potato psyllid's process so that it can be understood quickly and easily. Only a basic understanding of textbook molting events is needed, i.e. apolysis, molting space, secretion of new cuticle, and ecdysis. The animation also shows examples of key features of the potato psyllid's process that are present in ACP, indicating that both are comparable.


Citation: Cicero, JM, Alba-Tercedor, J, Hunter, WB, Saha, S and Brown, SJ. () An animated correspondence of Asian citrus psyllid stylets to the model for biogenesis of potato psyllid stylets. Developing an infrastructure and product test pipeline to deliver novel therapies for citrus greening disease. https://citrusgreening.org. Last viewed on